Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons. Academic Article uri icon

Overview

abstract

  • Accurate modeling of human neuronal cell biology has been a long-standing challenge. However, methods to differentiate human induced pluripotent stem cells (iPSCs) to neurons have recently provided experimentally tractable cell models. Numerous methods that use small molecules to direct iPSCs into neuronal lineages have arisen in recent years. Unfortunately, these methods entail numerous challenges, including poor efficiency, variable cell type heterogeneity, and lengthy, expensive differentiation procedures. We recently developed a new method to generate stable transgenic lines of human iPSCs with doxycycline-inducible transcription factors at safe-harbor loci. Using a simple two-step protocol, these lines can be inducibly differentiated into either cortical (i3 Neurons) or lower motor neurons (i3 LMN) in a rapid, efficient, and scalable manner (Wang et al., 2017). In this manuscript, we describe a set of protocols to assist investigators in the culture and genetic engineering of iPSC lines to enable transcription factor-mediated differentiation of iPSCs into i3 Neurons or i3 LMNs, and we present neuronal culture conditions for various experimental applications. © 2018 by John Wiley & Sons, Inc.

publication date

  • May 18, 2018

Research

keywords

  • Cell Culture Techniques
  • Cell Differentiation
  • Induced Pluripotent Stem Cells
  • Motor Neurons
  • Transcription Factors

Identity

PubMed Central ID

  • PMC6993937

Scopus Document Identifier

  • 85054061254

Digital Object Identifier (DOI)

  • 10.3791/1427

PubMed ID

  • 29924488

Additional Document Info

volume

  • 79

issue

  • 1