High-speed AFM height spectroscopy reveals µs-dynamics of unlabeled biomolecules. Academic Article uri icon

Overview

abstract

  • Dynamics are fundamental to the functions of biomolecules and can occur on a wide range of time and length scales. Here we develop and apply high-speed AFM height spectroscopy (HS-AFM-HS), a technique whereby we monitor the sensing of a HS-AFM tip at a fixed position to directly detect the motions of unlabeled molecules underneath. This gives Angstrom spatial and microsecond temporal resolutions. In conjunction with HS-AFM imaging modes to precisely locate areas of interest, HS-AFM-HS measures simultaneously surface concentrations, diffusion coefficients and oligomer sizes of annexin-V on model membranes to decipher key kinetics allowing us to describe the entire annexin-V membrane-association and self-assembly process in great detail and quantitatively. This work displays how HS-AFM-HS can assess the dynamics of unlabeled bio-molecules over several orders of magnitude and separate the various dynamic components spatiotemporally.

publication date

  • November 26, 2018

Research

keywords

  • Microscopy, Atomic Force
  • Spectrum Analysis
  • Staining and Labeling

Identity

PubMed Central ID

  • PMC6255864

Scopus Document Identifier

  • 85057195481

Digital Object Identifier (DOI)

  • 10.1063/1.5038095

PubMed ID

  • 30478320

Additional Document Info

volume

  • 9

issue

  • 1