Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast. Academic Article uri icon

Overview

abstract

  • Arrays of nucleosomes were positioned with respect to the GAL1-GAL10 intergenic region inserted into Saccharomyces cerevisiae minichromosomes. Deletions of DNA flanking the upstream activation sequence left the array unaltered, showing that nucleosome positioning was not a consequence of sequence-specific histone-DNA interactions but depended on proximity to the galactose-responsive upstream activation sequence (UASG). Replacement of the upstream activation sequence by synthetic oligonucleotides with different protein-binding properties identified a short sequence within this region that is responsible for the ordered array. This sequence overlaps a binding site for GAL4 protein, a positive regulator of transcription, but exerts its effect on chromatin structure independently of GAL4, probably through binding a novel factor that is not GAL-specific.

publication date

  • November 5, 1988

Research

keywords

  • Fungal Proteins
  • Nucleosomes
  • Saccharomyces cerevisiae

Identity

Scopus Document Identifier

  • 0024261880

Digital Object Identifier (DOI)

  • 10.1016/0022-2836(88)90603-1

PubMed ID

  • 3063825

Additional Document Info

volume

  • 204

issue

  • 1