Measurement of Intracellular Sterol Transport in Yeast. Academic Article uri icon

Overview

abstract

  • Intracellular sterol transport occurs largely by non-vesicular mechanisms in which sterol transport proteins extract sterol from one membrane and transfer it to another across the cytoplasm. Here we describe a suite of complementary assays to measure intracellular sterol transport in the model eukaryote Saccharomyces cerevisiae, as well as to quantify protein-mediated sterol transport between populations of vesicles in vitro. The in vivo assays can be adapted to study sterol transport in other cell types.

publication date

  • January 1, 2019

Research

keywords

  • Saccharomyces cerevisiae
  • Sterols

Identity

Scopus Document Identifier

  • 85061996237

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-9136-5_10

PubMed ID

  • 30790253

Additional Document Info

volume

  • 1949