selected publications
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An alliance between lipid transfer proteins and scramblases for membrane expansion.
Faculty reviews.
2022
Comment
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Times cited: 79 -
Single Vesicle Fluorescence-Bleaching Assay for Multi-Parameter Analysis of Proteoliposomes by Total Internal Reflection Fluorescence Microscopy.
ACS applied materials & interfaces.
2022
Academic Article
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Times cited: 2 - Cholesterol occupies the lipid translocation pathway to block phospholipid scrambling by a G protein-coupled receptor. Structure (London, England : 1993). 2022 Academic Article GET IT
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Genome-wide CRISPR screen reveals CLPTM1L as a lipid scramblase required for efficient glycosylphosphatidylinositol biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America.
2022
Academic Article
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Times cited: 2 -
Rethinking Opsins.
Molecular biology and evolution.
2022
Academic Article
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Times cited: 5 -
Identification of TbPBN1 in Trypanosoma brucei reveals a conserved heterodimeric architecture for glycosylphosphatidylinositol-mannosyltransferase-I.
Molecular microbiology.
2021
Academic Article
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Times cited: 1 -
Phospholipid Scrambling by G Protein-Coupled Receptors.
Annual review of biophysics.
2021
Review
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Times cited: 6 -
Elimination of GPI2 suppresses glycosylphosphatidylinositol GlcNAc transferase activity and alters GPI glycan modification in Trypanosoma brucei.
The Journal of biological chemistry.
2021
Academic Article
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Times cited: 3 -
Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.
Scientific reports.
2021
Academic Article
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Times cited: 2 -
Unusual mode of dimerization of retinitis pigmentosa-associated F220C rhodopsin.
Scientific reports.
2021
Academic Article
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Times cited: 4 -
Complexity of the eukaryotic dolichol-linked oligosaccharide scramblase suggested by activity correlation profiling mass spectrometry.
Scientific reports.
2021
Academic Article
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Times cited: 8 -
Nonenzymatic synthesis of anomerically pure, mannosyl-based molecular probes for scramblase identification studies.
Beilstein journal of organic chemistry.
2020
Academic Article
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Times cited: 1138 -
A PhotoClick cholesterol-based quantitative proteomics screen for cytoplasmic sterol-binding proteins in Saccharomyces cerevisiae.
Yeast (Chichester, England).
2020
Academic Article
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Times cited: 3 -
Exchange of water for sterol underlies sterol egress from a StARkin domain.
eLife.
2019
Academic Article
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Times cited: 10 -
Dysregulated calcium homeostasis prevents plasma membrane repair in Anoctamin 5/TMEM16E-deficient patient muscle cells.
Cell death discovery.
2019
Academic Article
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Times cited: 29178 -
Measurement of Intracellular Sterol Transport in Yeast.
Methods in molecular biology (Clifton, N.J.).
2019
Academic Article
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Times cited: 5 -
Scrambling of natural and fluorescently tagged phosphatidylinositol by reconstituted G protein-coupled receptor and TMEM16 scramblases.
The Journal of biological chemistry.
2018
Academic Article
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Times cited: 10 -
Gating mechanism of the extracellular entry to the lipid pathway in a TMEM16 scramblase.
Nature communications.
2018
Academic Article
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Times cited: 52 -
TOR complex 2-regulated protein kinase Ypk1 controls sterol distribution by inhibiting StARkin domain-containing proteins located at plasma membrane-endoplasmic reticulum contact sites.
Molecular biology of the cell.
2018
Academic Article
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Times cited: 22 -
Out-of-the-groove transport of lipids by TMEM16 and GPCR scramblases.
Proceedings of the National Academy of Sciences of the United States of America.
2018
Academic Article
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Times cited: 34 -
Structural mapping of fluorescently-tagged, functional nhTMEM16 scramblase in a lipid bilayer.
The Journal of biological chemistry.
2018
Academic Article
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Times cited: 7 -
Endoplasmic reticulum-plasma membrane contact sites integrate sterol and phospholipid regulation.
PLoS biology.
2018
Academic Article
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Times cited: 85 -
Sterol gradients in cells.
Current opinion in cell biology.
2018
Review
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Times cited: 24 -
Transbilayer lipid asymmetry.
Current biology : CB.
2018
Review
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Times cited: 81 -
Ergosterol is mainly located in the cytoplasmic leaflet of the yeast plasma membrane.
Traffic (Copenhagen, Denmark).
2018
Academic Article
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Times cited: 42 -
Structural basis of sterol binding and transport by a yeast StARkin domain.
The Journal of biological chemistry.
2018
Academic Article
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Times cited: 32 -
Mechanisms of Lipid Scrambling by the G Protein-Coupled Receptor Opsin.
Structure (London, England : 1993).
2017
Academic Article
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Times cited: 38 -
An engineered opsin monomer scrambles phospholipids.
Scientific reports.
2017
Academic Article
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Times cited: 10 -
Light-independent phospholipid scramblase activity of bacteriorhodopsin from Halobacterium salinarum.
Scientific reports.
2017
Academic Article
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Times cited: 15 -
Disulfide Bond Formation and N-Glycosylation Modulate Protein-Protein Interactions in GPI-Transamidase (GPIT).
Scientific reports.
2017
Academic Article
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Times cited: 9 - flippant-An R package for the automated analysis of fluorescence-based scramblase assays. BMC bioinformatics. 2017 Academic Article GET IT
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Speed Limits for Nonvesicular Intracellular Sterol Transport.
Trends in biochemical sciences.
2016
Review
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Times cited: 42 -
RFT1 Protein Affects Glycosylphosphatidylinositol (GPI) Anchor Glycosylation.
The Journal of biological chemistry.
2016
Academic Article
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Times cited: 9 -
The nhTMEM16 Scramblase Is Also a Nonselective Ion Channel.
Biophysical journal.
2016
Academic Article
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Times cited: 51 -
Dimerization deficiency of enigmatic retinitis pigmentosa-linked rhodopsin mutants.
Nature communications.
2016
Academic Article
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Times cited: 45 -
A Fluorescence-based Assay of Phospholipid Scramblase Activity.
Journal of visualized experiments : JoVE.
2016
Academic Article
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Times cited: 29 -
Lipid somersaults: Uncovering the mechanisms of protein-mediated lipid flipping.
Progress in lipid research.
2016
Review
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Times cited: 105 -
The cellular lipid landscape.
Biochimica et biophysica acta.
2016
Editorial Article
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Times cited: 4 -
Lipid topogenesis--35years on.
Biochimica et biophysica acta.
2016
Review
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Times cited: 17 -
Phospholipid scrambling by rhodopsin.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.
2015
Review
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Times cited: 31 -
Phosphatidylserine translocation at the yeast trans-Golgi network regulates protein sorting into exocytic vesicles.
Molecular biology of the cell.
2015
Academic Article
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Times cited: 46 -
Cell biology: Countercurrents in lipid flow.
Nature.
2015
Academic Article
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Times cited: 6 -
Structural biology: Lipid gymnastics.
Nature.
2015
Comment
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Times cited: 1 -
A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport.
eLife.
2015
Academic Article
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Times cited: 183 -
Constitutive phospholipid scramblase activity of a G protein-coupled receptor.
Nature communications.
2014
Academic Article
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Times cited: 87 -
Lipid landscapes and pipelines in membrane homeostasis.
Nature.
2014
Review
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Times cited: 575 -
A protein pair with PIPs inside.
Structure (London, England : 1993).
2013
Comment
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Times cited: 4 -
Arv1 regulates PM and ER membrane structure and homeostasis but is dispensable for intracellular sterol transport.
Traffic (Copenhagen, Denmark).
2013
Academic Article
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Times cited: 25 -
Glycoprotein biosynthesis in a eukaryote lacking the membrane protein Rft1.
The Journal of biological chemistry.
2013
Academic Article
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Times cited: 23 -
Ca2+-dependent phospholipid scrambling by a reconstituted TMEM16 ion channel.
Nature communications.
2013
Academic Article
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Times cited: 172 -
Reconstitution of glucosylceramide flip-flop across endoplasmic reticulum: implications for mechanism of glycosphingolipid biosynthesis.
The Journal of biological chemistry.
2012
Academic Article
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Times cited: 32 -
A detour for yeast oxysterol binding proteins.
The Journal of biological chemistry.
2012
Review
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Times cited: 56 -
Osh proteins regulate membrane sterol organization but are not required for sterol movement between the ER and PM.
Traffic (Copenhagen, Denmark).
2011
Academic Article
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Times cited: 100 -
Opsin is a phospholipid flippase.
Current biology : CB.
2011
Academic Article
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Times cited: 118 -
Stereoselective transbilayer translocation of mannosyl phosphoryl dolichol by an endoplasmic reticulum flippase.
Proceedings of the National Academy of Sciences of the United States of America.
2010
Academic Article
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Times cited: 40 -
Flipping lipids: why an' what's the reason for?.
ACS chemical biology.
2009
Review
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Times cited: 102 -
Specific transbilayer translocation of dolichol-linked oligosaccharides by an endoplasmic reticulum flippase.
Proceedings of the National Academy of Sciences of the United States of America.
2009
Academic Article
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Times cited: 49 -
Yeast oxysterol-binding proteins: sterol transporters or regulators of cell polarization?.
Molecular and cellular biochemistry.
2009
Academic Article
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Times cited: 23 -
Tritium suicide selection identifies proteins involved in the uptake and intracellular transport of sterols in Saccharomyces cerevisiae.
Eukaryotic cell.
2008
Academic Article
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Times cited: 23 -
Does Rft1 flip an N-glycan lipid precursor?.
Nature.
2008
Comment
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Times cited: 58 -
Distinct flippases translocate glycerophospholipids and oligosaccharide diphosphate dolichols across the endoplasmic reticulum.
Biochemistry.
2008
Academic Article
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Times cited: 71 -
A flip-flop switch in polarity signaling.
Developmental cell.
2007
Comment
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Times cited: 3 -
Candida drug resistance protein 1, a major multidrug ATP binding cassette transporter of Candida albicans, translocates fluorescent phospholipids in a reconstituted system.
Biochemistry.
2007
Academic Article
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Times cited: 22 -
Flip-flop of fluorescently labeled phospholipids in proteoliposomes reconstituted with Saccharomyces cerevisiae microsomal proteins.
Eukaryotic cell.
2007
Academic Article
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Times cited: 37 -
HeLa cell entry by guanidinium-rich beta-peptides: importance of specific cation-cell surface interactions.
Chembiochem : a European journal of chemical biology.
2007
Academic Article
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Times cited: 43 -
Thematic review series: lipid posttranslational modifications. GPI anchoring of protein in yeast and mammalian cells, or: how we learned to stop worrying and love glycophospholipids.
Journal of lipid research.
2007
Review
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Times cited: 326 -
De novo sphingolipid synthesis is essential for viability, but not for transport of glycosylphosphatidylinositol-anchored proteins, in African trypanosomes.
Eukaryotic cell.
2007
Academic Article
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Times cited: 39 -
Lipid flippases and their biological functions.
Cellular and molecular life sciences : CMLS.
2006
Review
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Times cited: 225 -
Ethanolamine phosphate linked to the first mannose residue of glycosylphosphatidylinositol (GPI) lipids is a major feature of the GPI structure that is recognized by human GPI transamidase.
The Journal of biological chemistry.
2006
Academic Article
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Times cited: 14 -
Intracellular sterol transport and distribution.
Current opinion in cell biology.
2006
Review
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Times cited: 104 -
Sterol trafficking between the endoplasmic reticulum and plasma membrane in yeast.
Biochemical Society transactions.
2006
Review
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Times cited: 74 -
Regulation of surface coat exchange by differentiating African trypanosomes.
Molecular and biochemical parasitology.
2006
Academic Article
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Times cited: 42 -
Transport of newly synthesized sterol to the sterol-enriched plasma membrane occurs via nonvesicular equilibration.
Biochemistry.
2005
Academic Article
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Times cited: 172 -
Effects of conformational stability and geometry of guanidinium display on cell entry by beta-peptides.
Journal of the American Chemical Society.
2005
Academic Article
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Times cited: 88 -
New fluorescent probes reveal that flippase-mediated flip-flop of phosphatidylinositol across the endoplasmic reticulum membrane does not depend on the stereochemistry of the lipid.
Organic & biomolecular chemistry.
2005
Academic Article
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Times cited: 58 -
Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex.
The Journal of biological chemistry.
2005
Academic Article
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Times cited: 9 -
Flip-flop of glycosylphosphatidylinositols (GPI's) across the ER.
Chemical communications (Cambridge, England).
2004
Academic Article
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Times cited: 51 -
Chemical modification identifies two populations of glycerophospholipid flippase in rat liver ER.
Biochemistry.
2004
Academic Article
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Times cited: 55 -
Subcellular localization and targeting of N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the second enzyme in the glycosylphosphatidylinositol biosynthetic pathway.
The Journal of biological chemistry.
2004
Academic Article
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Times cited: 21 -
A conserved proline in the last transmembrane segment of Gaa1 is required for glycosylphosphatidylinositol (GPI) recognition by GPI transamidase.
The Journal of biological chemistry.
2003
Academic Article
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Times cited: 34 -
Comparative importance in vivo of conserved glutamate residues in the EX7E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit of the first enzyme in glycosylphosphatidylinositol assembly.
European journal of biochemistry.
2003
Academic Article
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Times cited: 18 -
Cytoplasmic and nuclear delivery of a TAT-derived peptide and a beta-peptide after endocytic uptake into HeLa cells.
The Journal of biological chemistry.
2003
Academic Article
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Times cited: 203 -
Reconstitution and assay of biogenic membrane-derived phospholipid flippase activity in proteoliposomes.
Methods in molecular biology (Clifton, N.J.).
2003
Academic Article
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Times cited: 11 -
Reconstitution of phospholipid flippase activity from E. coli inner membrane: a test of the protein translocon as a candidate flippase.
Biological chemistry.
2002
Academic Article
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Times cited: 11 -
Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex.
The Journal of biological chemistry.
2002
Academic Article
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Times cited: 40 -
Introduction: lipid transport--an overview.
Seminars in cell & developmental biology.
2002
Academic Article
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Times cited: 5 -
Transbilayer movement of dipalmitoylphosphatidylcholine in proteoliposomes reconstituted from detergent extracts of endoplasmic reticulum. Kinetics of transbilayer transport mediated by a single flippase and identification of protein fractions enriched in flippase activity.
The Journal of biological chemistry.
2002
Academic Article
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Times cited: 46 -
Transbilayer movement of fluorescent phospholipid analogues in the cytoplasmic membrane of Escherichia coli.
Biochemistry.
2002
Academic Article
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Times cited: 41 -
Endoplasmic reticulum proteins involved in glycosylphosphatidylinositol-anchor attachment: photocrosslinking studies in a cell-free system.
European journal of biochemistry.
2001
Academic Article
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Times cited: 32 -
The DXD motif is required for GM2 synthase activity but is not critical for nucleotide binding.
Glycobiology.
2001
Academic Article
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Times cited: 28 -
Soluble GPI8 restores glycosylphosphatidylinositol anchoring in a trypanosome cell-free system depleted of lumenal endoplasmic reticulum proteins.
The Biochemical journal.
2000
Academic Article
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Times cited: 30 -
Photoaffinity labelling with P3-(4-azidoanilido)uridine 5'-triphosphate identifies gpi3p as the UDP-GlcNAc-binding subunit of the enzyme that catalyses formation of GlcNAc-phosphatidylinositol, the first glycolipid intermediate in glycosylphosphatidylinositol synthesis.
The Biochemical journal.
2000
Academic Article
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Times cited: 37 -
Reconstitution and partial characterization of phospholipid flippase activity from detergent extracts of the Bacillus subtilis cell membrane.
Journal of bacteriology.
2000
Academic Article
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Times cited: 45 -
Cell surface display and intracellular trafficking of free glycosylphosphatidylinositols in mammalian cells.
The Journal of biological chemistry.
2000
Academic Article
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Times cited: 42 -
Specific proteins are required to translocate phosphatidylcholine bidirectionally across the endoplasmic reticulum.
Current biology : CB.
2000
Academic Article
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Times cited: 81 -
Recent developments in the cell biology and biochemistry of glycosylphosphatidylinositol lipids (review).
Molecular membrane biology.
2000
Review
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Times cited: 127 -
A cell-free assay for glycosylphosphatidylinositol anchoring in African trypanosomes. Demonstration of a transamidation reaction mechanism.
The Journal of biological chemistry.
1999
Academic Article
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Times cited: 60 -
Segregation of glycosylphosphatidylinositol biosynthetic reactions in a subcompartment of the endoplasmic reticulum.
The Journal of biological chemistry.
1999
Academic Article
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Times cited: 55 -
Identification and purification of the rat liver Golgi membrane UDP-N-acetylgalactosamine transporter.
The Journal of biological chemistry.
1999
Academic Article
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Times cited: 46 -
Lipids: more than just membrane fabric.
Trends in cell biology.
1998
Conference Paper
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Times cited: 6 -
Identification of endoplasmic reticulum proteins involved in glycan assembly: synthesis and characterization of P3-(4-azidoanilido)uridine 5'-triphosphate, a membrane-topological photoaffinity probe for uridine diphosphate-sugar binding proteins.
The Biochemical journal.
1998
Academic Article
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Times cited: 4 -
Glycosylphosphatidylinositols: biosynthesis and intracellular transport.
Biochemical Society transactions.
1997
Review
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Times cited: 17 -
Transbilayer movement of fluorescent phospholipids in Bacillus megaterium membrane vesicles.
Biochemistry.
1997
Academic Article
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Times cited: 58 -
Phosphatidylinositol hydrolysis by Trypanosoma brucei glycosylphosphatidylinositol phospholipase C.
The Journal of biological chemistry.
1996
Academic Article
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Times cited: 36 -
Nonpolarized distribution of glycosylphosphatidylinositols in the plasma membrane of polarized Madin-Darby canine kidney cells.
The Journal of biological chemistry.
1995
Academic Article
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Times cited: 37 -
Soluble constituents of the ER lumen are required for GPI anchoring of a model protein.
The EMBO journal.
1995
Academic Article
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Times cited: 25 -
Flippases.
Trends in cell biology.
1995
Academic Article
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Times cited: 76 -
Production of a nested set of glycosylphosphatidylinositol structures from a glycosylphosphatidylinositol-anchored protein.
Analytical biochemistry.
1995
Academic Article
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Times cited: 9 -
Biosynthesis of glycosylphosphatidylinositol anchors.
Methods in enzymology.
1995
Academic Article
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Times cited: 19 -
The GPI anchor of cell-surface proteins is synthesized on the cytoplasmic face of the endoplasmic reticulum.
The Journal of cell biology.
1994
Academic Article
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Times cited: 97 -
Topology of GPI biosynthesis in the endoplasmic reticulum.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.
1994
Academic Article
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Times cited: 8 -
Structural analysis of glycosylphosphatidylinositol anchors.
Methods in enzymology.
1994
Academic Article
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Times cited: 37 -
Early lipid intermediates in glycosyl-phosphatidylinositol anchor assembly are synthesized in the ER and located in the cytoplasmic leaflet of the ER membrane bilayer.
The Journal of cell biology.
1993
Academic Article
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Times cited: 120 -
Phosphatidylethanolamine is the donor of the terminal phosphoethanolamine group in trypanosome glycosylphosphatidylinositols.
The EMBO journal.
1993
Academic Article
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Times cited: 82 -
Molecular species analysis of phospholipids from Trypanosoma brucei bloodstream and procyclic forms.
Molecular and biochemical parasitology.
1993
Academic Article
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Times cited: 65 -
Complexity of ethanolamine phosphate addition in the biosynthesis of glycosylphosphatidylinositol anchors in mammalian cells.
The Journal of biological chemistry.
1992
Academic Article
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Times cited: 62 -
Phosphatidylethanolamine is the donor of the ethanolamine residue linking a glycosylphosphatidylinositol anchor to protein.
The Journal of biological chemistry.
1992
Academic Article
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Times cited: 120 -
Developmental variation of glycosylphosphatidylinositol membrane anchors in Trypanosoma brucei. In vitro biosynthesis of intermediates in the construction of the GPI anchor of the major procyclic surface glycoprotein.
The Journal of biological chemistry.
1992
Academic Article
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Times cited: 35 -
Galactose-containing glycosylphosphatidylinositols in Trypanosoma brucei.
The Journal of biological chemistry.
1992
Academic Article
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Times cited: 34 -
Biosynthesis of glycosyl-phosphatidylinositol.
Cell biology international reports.
1991
Review
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Times cited: 9 -
The mevalonate pathway in the bloodstream form of Trypanosoma brucei. Identification of dolichols containing 11 and 12 isoprene residues.
The Journal of biological chemistry.
1991
Academic Article
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Times cited: 60 -
A glycosylphosphatidylinositol protein anchor from procyclic stage Trypanosoma brucei: lipid structure and biosynthesis.
The EMBO journal.
1991
Academic Article
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Times cited: 87 -
Mannosamine, a novel inhibitor of glycosylphosphatidylinositol incorporation into proteins.
The EMBO journal.
1991
Academic Article
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Times cited: 65 -
Transfer of glycosyl-phosphatidylinositol membrane anchors to polypeptide acceptors in a cell-free system.
The Journal of cell biology.
1991
Academic Article
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Times cited: 53 -
Developmental variation of glycosylphosphatidylinositol membrane anchors in Trypanosoma brucei. Identification of a candidate biosynthetic precursor of the glycosylphosphatidylinositol anchor of the major procyclic stage surface glycoprotein.
The Journal of biological chemistry.
1991
Academic Article
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Times cited: 46 -
Biosynthesis of glycosyl-phosphatidylinositol lipids in Trypanosoma brucei: involvement of mannosyl-phosphoryldolichol as the mannose donor.
The EMBO journal.
1990
Academic Article
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Times cited: 123 -
Cell-free synthesis of glycosyl-phosphatidylinositol precursors for the glycolipid membrane anchor of Trypanosoma brucei variant surface glycoproteins. Structural characterization of putative biosynthetic intermediates.
The Journal of biological chemistry.
1990
Academic Article
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Times cited: 109 -
Glycolipid precursors for the membrane anchor of Trypanosoma brucei variant surface glycoproteins. I. Can structure of the phosphatidylinositol-specific phospholipase C sensitive and resistant glycolipids.
The Journal of biological chemistry.
1990
Academic Article
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Times cited: 64 -
Glycolipid precursors for the membrane anchor of Trypanosoma brucei variant surface glycoproteins. II. Lipid structures of phosphatidylinositol-specific phospholipase C sensitive and resistant glycolipids.
The Journal of biological chemistry.
1990
Academic Article
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Times cited: 66 -
Candidate glycophospholipid precursor for the glycosylphosphatidylinositol membrane anchor of Trypanosoma brucei variant surface glycoproteins.
The Journal of biological chemistry.
1988
Academic Article
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Times cited: 87 -
Clustering, mobility, and triggering activity of small oligomers of immunoglobulin E on rat basophilic leukemia cells.
The Journal of cell biology.
1986
Academic Article
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Times cited: 50 -
Cross-linking of receptor-bound IgE to aggregates larger than dimers leads to rapid immobilization.
The Journal of cell biology.
1986
Academic Article
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Times cited: 87 -
Small oligomers of immunoglobulin E (IgE) cause large-scale clustering of IgE receptors on the surface of rat basophilic leukemia cells.
The Journal of cell biology.
1984
Academic Article
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Times cited: 42