Transcriptome-Wide Mapping of m6 A and m6 Am at Single-Nucleotide Resolution Using miCLIP. Academic Article uri icon

Overview

abstract

  • The most prevalent modified base in mRNA, N6 -methyladenosine (m6 A), is found in several thousand transcripts, typically near the stop codon, although it can occur anywhere in the mRNA. In addition, the highly similar nucleotide N6 ,2'-O-dimethyladenosine (m6 Am), which is difficult to distinguish from m6 A, occurs as the first transcribed nucleotide of certain transcripts. Both the m6 A and m6 Am modifications have been implicated in numerous biological processes, and their precise mapping is crucial to understanding their functions. To address this need, we developed miCLIP, a method that maps both m6 A and m6 Am at individual nucleotide resolution. Here we describe the miCLIP protocol, with slight improvements to the initially published protocol for both the experimental methodology and bioinformatics analysis. © 2019 by John Wiley & Sons, Inc.

publication date

  • March 15, 2019

Research

keywords

  • Adenosine
  • Chemistry Techniques, Analytical
  • Molecular Biology
  • RNA, Messenger
  • Transcriptome

Identity

PubMed Central ID

  • PMC6422687

Scopus Document Identifier

  • 85063009642

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2016.01.012

PubMed ID

  • 30874375

Additional Document Info

volume

  • 126

issue

  • 1