Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling. Academic Article uri icon

Overview

abstract

  • Type I interferons (IFNs) play a critical role in host defense against influenza virus infection, and the mechanism of influenza virus to evade type I IFNs responses remains to be fully understood. Here, we found that progranulin (PGRN) was significantly increased both in vitro and in vivo during influenza virus infection. Using a PGRN knockdown assay and PGRN-deficient mice model, we demonstrated that influenza virus-inducing PGRN negatively regulated type I IFNs production by inhibiting the activation of NF-κB and IRF3 signaling. Furthermore, we showed that PGRN directly interacted with NF-κB essential modulator (NEMO) via its Grn CDE domains. We also verified that PGRN recruited A20 to deubiquitinate K63-linked polyubiquitin chains on NEMO at K264. In addition, we found that macrophage played a major source of PGRN during influenza virus infection, and PGRN neutralizing antibodies could protect against influenza virus-induced lethality in mice. Our data identify a PGRN-mediated IFN evasion pathway exploited by influenza virus with implication in antiviral applications. These findings also provide insights into the functions and crosstalk of PGRN in innate immunity.

publication date

  • October 4, 2019

Research

keywords

  • Antiviral Agents
  • Immune Evasion
  • Immunity, Innate
  • Interferon Type I
  • Orthomyxoviridae
  • Orthomyxoviridae Infections
  • Progranulins

Identity

PubMed Central ID

  • PMC6795447

Scopus Document Identifier

  • 85073488012

Digital Object Identifier (DOI)

  • 10.1002/art.34664

PubMed ID

  • 31585000

Additional Document Info

volume

  • 15

issue

  • 10