Engineering transferrable microvascular meshes for subcutaneous islet transplantation. Academic Article uri icon

Overview

abstract

  • The success of engineered cell or tissue implants is dependent on vascular regeneration to meet adequate metabolic requirements. However, development of a broadly applicable strategy for stable and functional vascularization has remained challenging. We report here highly organized and resilient microvascular meshes fabricated through a controllable anchored self-assembly method. The microvascular meshes are scalable to centimeters, almost free of defects and transferrable to diverse substrates, ready for transplantation. They promote formation of functional blood vessels, with a density as high as ~220 vessels mm-2, in the poorly vascularized subcutaneous space of SCID-Beige mice. We further demonstrate the feasibility of fabricating microvascular meshes from human induced pluripotent stem cell-derived endothelial cells, opening a way to engineer patient-specific microvasculature. As a proof-of-concept for type 1 diabetes treatment, we combine microvascular meshes and subcutaneously transplanted rat islets and achieve correction of chemically induced diabetes in SCID-Beige mice for 3 months.

publication date

  • October 10, 2019

Research

keywords

  • Cell Culture Techniques
  • Diabetes Mellitus, Experimental
  • Islets of Langerhans Transplantation
  • Microvessels

Identity

PubMed Central ID

  • PMC6787187

Scopus Document Identifier

  • 85073099809

Digital Object Identifier (DOI)

  • 10.1089/ten.2006.12.2875

PubMed ID

  • 31601796

Additional Document Info

volume

  • 10

issue

  • 1