Nonenzymatic synthesis of anomerically pure, mannosyl-based molecular probes for scramblase identification studies. Academic Article uri icon

Overview

abstract

  • The chemical synthesis of molecular probes to identify and study membrane proteins involved in the biological pathway of protein glycosylation is described. Two short-chain glycolipid analogs that mimic the naturally occurring substrate mannosyl phosphoryl dolichol exhibit either photoreactive and clickable properties or allow the use of a fluorescence readout. Both probes consist of a hydrophilic mannose headgroup that is linked to a citronellol derivative via a phosphodiester bridge. Moreover, a novel phosphoramidite chemistry-based method offers a straightforward approach for the non-enzymatic incorporation of the saccharide moiety in an anomerically pure form.

publication date

  • July 20, 2020

Identity

PubMed Central ID

  • PMC7385334

Scopus Document Identifier

  • 4244144203

Digital Object Identifier (DOI)

  • 10.1039/p29740000293

PubMed ID

  • 32765793

Additional Document Info

volume

  • 16