A MAC2-positive progenitor-like microglial population is resistant to CSF1R inhibition in adult mouse brain. Academic Article uri icon

Overview

abstract

  • Microglia are the resident myeloid cells in the central nervous system (CNS). The majority of microglia rely on CSF1R signaling for survival. However, a small subset of microglia in mouse brains can survive without CSF1R signaling and reestablish the microglial homeostatic population after CSF1R signaling returns. Using single-cell transcriptomic analysis, we characterized the heterogeneous microglial populations under CSF1R inhibition, including microglia with reduced homeostatic markers and elevated markers of inflammatory chemokines and proliferation. Importantly, MAC2/Lgals3 was upregulated under CSF1R inhibition, and shared striking similarities with microglial progenitors in the yolk sac and immature microglia in early embryos. Lineage-tracing studies revealed that these MAC2+ cells were of microglial origin. MAC2+ microglia were also present in non-treated adult mouse brains and exhibited immature transcriptomic signatures indistinguishable from those that survived CSF1R inhibition, supporting the notion that MAC2+ progenitor-like cells are present among adult microglia.

publication date

  • October 15, 2020

Research

keywords

  • Brain
  • Galectin 3
  • Mice
  • Microglia
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
  • Signal Transduction

Identity

PubMed Central ID

  • PMC7591254

Scopus Document Identifier

  • 85094933202

Digital Object Identifier (DOI)

  • 10.1038/s41467-018-06224-y

PubMed ID

  • 33054973

Additional Document Info

volume

  • 9