Single-Cell Sorting of HBsAg-Binding Memory B Cells from Human Peripheral Blood Mononuclear Cells and Antibody Cloning. Academic Article uri icon

Overview

abstract

  • The isolation of human antibodies with naturally paired heavy and light chains is crucial for understanding the human antibody immune response. Here, we present a protocol for antibody cloning from the sorted single human memory B cells recognizing hepatitis B virus (HBV) S antigen (HBsAg). A two-fluorescent-dye labeling strategy against HBsAg allows for an improved sorting specificity, while non-relevant protein staining allows for the exclusion of non-specific B cells. This protocol could also be widely adapted for other antigens. For complete details on the use and execution of this protocol, please refer to Wang et al. (2020).

publication date

  • October 16, 2020

Research

keywords

  • Flow Cytometry
  • Memory B Cells

Identity

PubMed Central ID

  • PMC7757113

Scopus Document Identifier

  • 85108297643

Digital Object Identifier (DOI)

  • 10.1016/j.chom.2020.05.010

PubMed ID

  • 33377023

Additional Document Info

volume

  • 1

issue

  • 3