Mouse model of colorectal cancer: orthotopic co-implantation of tumor and stroma cells in cecum and rectum. Academic Article uri icon

Overview

abstract

  • In vivo interrogation of the functional role of genes implicated in colorectal cancer (CRC) is limited by the need for physiological models that mimic the disease. Here, we describe a protocol that provides the steps required for the orthotopic co-implantation of tumoral and stromal cells into the cecum and rectum to investigate the crosstalk between the tumor and its microenvironment. This protocol recapitulates metastases to the lymph nodes, liver, and lungs observed in human CRC. For complete details on the use and execution of this protocol, please refer to Kasashima et al. (2020).

publication date

  • January 22, 2021

Research

keywords

  • Cecum
  • Colorectal Neoplasms
  • Neoplasms, Experimental
  • Rectum
  • Tumor Microenvironment

Identity

PubMed Central ID

  • PMC7843928

Scopus Document Identifier

  • 85122812999

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2020.10.014

PubMed ID

  • 33554135

Additional Document Info

volume

  • 2

issue

  • 1