Isolation and Stimulation of Peritoneal Macrophages withApoptotic Jurkat Cells to Produce IL-10. Academic Article uri icon

Overview

abstract

  • Clearance of apoptotic cells by macrophages is critical to ensuring cellular homeostasis and suppression of autoimmunity. Macrophage recognition of apoptotic cells triggers an anti-inflammatory response, which is mediated by the release of IL-10, TGF-β etc. with concurrent inhibition of pro-inflammatory cytokines (such as TNFα, IL-12, IL-1β). To characterize cytokine profile produced by macrophages during phagocytosis of apoptotic cells, we developed an effective, more physiologic system using isolated murine peritoneal macrophages and T-lymphocyte cell line Jurkat as a source of apoptotic cells. Apoptosis of Jurkat cells is induced with staurosporine, a protein kinase C (PKC) inhibitor and detected by Annexin V/propidium iodide staining. This in vitro assay demonstrates that murine peritoneal macrophages produce large amounts of IL-10 following exposure to apoptotic Jurkat cells.

publication date

  • December 20, 2019

Identity

PubMed Central ID

  • PMC7853933

Scopus Document Identifier

  • 85138273530

Digital Object Identifier (DOI)

  • 10.21769/BioProtoc.3467

PubMed ID

  • 33654958

Additional Document Info

volume

  • 9

issue

  • 24