Activity-based annotation: the emergence of systems biochemistry. Review uri icon

Overview

abstract

  • Current tools to annotate protein function have failed to keep pace with the speed of DNA sequencing and exponentially growing number of proteins of unknown function (PUFs). A major contributing factor to this mismatch is the historical lack of high-throughput methods to experimentally determine biochemical activity. Activity-based methods, such as activity-based metabolite and protein profiling, are emerging as new approaches for unbiased, global, biochemical annotation of protein function. In this review, we highlight recent experimental, activity-based approaches that offer new opportunities to determine protein function in a biologically agnostic and systems-level manner.

publication date

  • April 13, 2022

Identity

PubMed Central ID

  • PMC9378515

Scopus Document Identifier

  • 85128244725

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2017.12.006

PubMed ID

  • 35430135

Additional Document Info

volume

  • 47

issue

  • 9