Air-liquid organotypic assays to investigate cellular crosstalk in the tumor microenvironment of cancer cells. Academic Article uri icon

Overview

abstract

  • Air-liquid organotypic culture models enable the study of the cellular crosstalk in the tumor microenvironment. This 3D assay recapitulates the tumor niche more faithfully than 2D culture systems and represents a versatile platform that can be easily adapted to different types of cancer cells, stromal components, or ECM composition. Here, we detail the steps to build an organotypic culture including the preparation of the organotypic structure, organotypic gels, cell seeding, gel casting, membrane processing, and image and data analysis. For complete details on the use and execution of this protocol, please refer to Linares et al. (2022).

publication date

  • August 18, 2022

Research

keywords

  • Neoplasms
  • Tumor Microenvironment

Identity

PubMed Central ID

  • PMC9405995

Scopus Document Identifier

  • 85136209548

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2022.101635

PubMed ID

  • 36035805

Additional Document Info

volume

  • 3

issue

  • 3