Protocol for Plasma Extracellular Vesicle and Particle Isolation and Mass Spectrometry-Based Proteomic Identification. Academic Article uri icon

Overview

abstract

  • Plasma extracellular vesicles and particles (EVPs) are enriched in biomolecules that reflect individuals' physiological and pathological states. Several studies have demonstrated the potential of human plasma EVPs as a novel liquid biopsy. Here we describe a protocol for human plasma EVPs isolation and proteomic characterization. We isolated human plasma EVPs by the classical ultracentrifugation method and performed mass spectrometry-based proteomic profiling. Using this protocol, researchers can reveal the plasma EVPs proteome and explore the clinical application of plasma EVPs proteins for developing disease biomarkers.

publication date

  • January 1, 2023

Research

keywords

  • Extracellular Vesicles
  • Proteomics

Identity

Scopus Document Identifier

  • 85147950399

Digital Object Identifier (DOI)

  • 10.1038/s41596-019-0236-5

PubMed ID

  • 36781793

Additional Document Info

volume

  • 2628