Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures. Academic Article uri icon

Overview

abstract

  • Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining in intact colorectal cancer organoids derived from mice. We also describe additional steps for co-culture with mouse fibroblasts to enable the study of interactions with other cellular components of the tissue microenvironment. For complete details on the use and execution of this protocol, please refer to Martinez-OrdoƱez et al. (2023).1.

publication date

  • April 20, 2023

Identity

PubMed Central ID

  • PMC10323124

Scopus Document Identifier

  • 85086325903

Digital Object Identifier (DOI)

  • 10.3791/60709

PubMed ID

  • 37083323

Additional Document Info

volume

  • 4

issue

  • 2