Rearrangement at the 5' end of amplified c-myc in human COLO 320 cells is associated with abnormal transcription. Academic Article uri icon

Overview

abstract

  • The proto-oncogene c-myc is amplified in sublines of human COLO 320 cells carrying either homogeneously staining chromosomal regions or double minutes. COLO 320 cells carrying homogeneously staining chromosomal regions have 15 to 20 copies of an apparently normal c-myc allele and 1 to 2 copies of an abnormal c-myc allele lacking exon 1 and express high levels of a normal c-myc mRNA 2.5 kilobases in size. COLO 320 cells carrying double minutes have about 25 copies each of the normal allele and the abnormal allele but express preferentially an abnormal c-myc mRNA 2.2 kilobases in size. Nucleotide sequence analyses revealed that the break point of rearrangement resulting in the loss of exon 1 in the abnormal allele lies within a region frequently rearranged in human and murine B-cell tumors.

publication date

  • July 1, 1986

Research

keywords

  • Gene Amplification
  • Oncogenes
  • Transcription, Genetic

Identity

PubMed Central ID

  • PMC367836

Scopus Document Identifier

  • 0022751357

Digital Object Identifier (DOI)

  • 10.1128/mcb.6.7.2752-2755.1986

PubMed ID

  • 3785211

Additional Document Info

volume

  • 6

issue

  • 7