Selective utilization of pyrimidine deoxyribonucleosides for deoxyribonucleic acid synthesis in pneumococcus. Academic Article uri icon

Overview

abstract

  • When pyrimidine deoxyribonucleosides are supplied to growing cultures of Diplococcus pneumoniae, they are selectively used for incorporation into deoxyribonucleic acid (DNA). Differently labeled molecules of deoxyuridine, thymidine, and deoxycytidine were used to study the precursor pathways of this organism. Each of these preformed pyrimidine deoxynucleosides is incorporated intact (i.e., without cleavage of the glycosidic bond) and is predominantly recoverable as DNA thymidine. During the utilization of deoxycytidine and deoxyuridine by pneumococci, large proportions of the available precursor are converted to free thymidine, which is secreted back into the growth medium. The biochemical pathways for selective incorporation into DNA and the regulation of concentrations of intracellular thymidine compounds by excretion of free thymidine are discussed.

publication date

  • March 1, 1973

Research

keywords

  • DNA, Bacterial
  • Deoxyribonucleosides
  • Streptococcus pneumoniae

Identity

PubMed Central ID

  • PMC251705

Scopus Document Identifier

  • 0015597125

Digital Object Identifier (DOI)

  • 10.1128/jb.113.3.1356-1362.1973

PubMed ID

  • 4144169

Additional Document Info

volume

  • 113

issue

  • 3