alpha-Actinin and membrane glycoprotein IIIa are different proteins in human blood platelets. Academic Article uri icon

Overview

abstract

  • It has been suggested that a platelet protein that is very similar to muscle alpha-actinin is identical to the membrane glycoprotein IIIa (GPIIIa) of platelets and is responsible for anchoring actin filaments directly into the plasma membrane of platelets. To determine if alpha-actinin and GPIIIa are related in platelets, we analyzed the purified proteins on 5% sodium dodecyl sulfate/polyacrylamide gels. The two proteins differ in mobility in both the unreduced and reduced states, and they stain differently with silver stain. In addition, alpha-actinin is a prominent component of the detergent-insoluble cytoskeletons of platelets, whereas GPIIIa is absent from these structures. By using monospecific antisera to the individual proteins, it was demonstrated that alpha-actinin and GPIIIa are immunologically distinct. We conclude that alpha-actinin and GPIIIa are different proteins in human blood platelets and that it is unlikely that alpha-actinin is an integral membrane protein.

publication date

  • January 1, 1982

Research

keywords

  • Actinin
  • Blood Platelets
  • Glycoproteins
  • Muscle Proteins

Identity

PubMed Central ID

  • PMC345757

Scopus Document Identifier

  • 0020032612

Digital Object Identifier (DOI)

  • 10.1073/pnas.79.2.432

PubMed ID

  • 6176993

Additional Document Info

volume

  • 79

issue

  • 2