Nucleotide sequence of the 5' noncoding region and part of the gag gene of Rous sarcoma virus. Academic Article uri icon

Overview

abstract

  • Several functions of the retrovirus genome involve structural features in the vicinity of its 5' terminus. In an effort to further elucidate the relationship between structure and function in retrovirus RNA, we have determined the sequence of the first 1,010 nucleotides at the 5' end of the genome of Rous sarcoma virus by using the Maxam-Gilbert method to sequence suitable domains in cloned Rous sarcoma virus DNA. The results (i) locate the initiation codon for the gag gene of Rous sarcoma virus 372 nucleotides from the 5' end of viral RNA; (ii) demonstrate that this codon is preceded by three methionine codons that are apparently not used in translation; (iii) sustain previous conclusions that the principal site to which ribosomes bind on the Rous sarcoma virus genome in vitro does not contain the initiation codon for gag; (iv) permit deduction of the amino acid sequence of a viral structural protein, p19; (v) confirm the amino-terminal sequence of Pr76gag; and (vi) substantiate the identification of a splice donor site described in the accompanying manuscript (Hackett et al., J. Virol., 41:527-534, 1982).

publication date

  • February 1, 1982

Research

keywords

  • Avian Sarcoma Viruses
  • RNA, Messenger
  • RNA, Viral
  • Viral Proteins

Identity

PubMed Central ID

  • PMC256782

Scopus Document Identifier

  • 0020069573

Digital Object Identifier (DOI)

  • 10.1128/JVI.41.2.535-541.1982

PubMed ID

  • 6281465

Additional Document Info

volume

  • 41

issue

  • 2