Heterogeneity of type 1 skeletal muscle fibers revealed by monoclonal antibody to slow myosin. Academic Article uri icon

Overview

abstract

  • Monoclonal antibodies (McAbs) were generated against slow myosin from the chicken anterior latissimus dorsi (ALD) muscle and their reactivity was checked against fast (pectoralis), slow (ALD), cardiac (ventricular), and smooth (gizzard) myosins by radioimmunoassay (RIA), immunoautoradiography (immunoblots), and indirect immunofluorescence techniques. In RIAs, the McAb (ALD-58) described in this article reacted specifically with slow myosin, with only a weak cross-reactivity to cardiac myosin. In immunoblots against whole muscle homogenates and purified myosins, it bound selectively to the 200 Kd myosin heavy chain band. The ALD-58 antibody stained the fibers of the ALD muscle uniformly but gave three grades of reactions (strong, weak, and negative), with histochemically identified type 1 fibers of sartorius and gastrocnemius muscles demonstrating the immunological heterogeneity of myosins in type 1 skeletal muscle fibers.

publication date

  • June 1, 1984

Research

keywords

  • Antibodies, Monoclonal
  • Muscles
  • Myosins

Identity

Scopus Document Identifier

  • 0021239713

Digital Object Identifier (DOI)

  • 10.1002/mus.880070507

PubMed ID

  • 6738576

Additional Document Info

volume

  • 7

issue

  • 5