Transcription and translation of inducible nitric oxide synthase in the pancreas of prediabetic BB rats. Academic Article uri icon

Overview

abstract

  • The inducible NO synthase (iNOS) was found to be expressed in pancreatic lesions of adult diabetes-prone BB rats. Pancreatic iNOS mRNA was detected by reverse transcriptase PCR in pancreatic RNA of adult diabetes-prone BB rats but not in normal Wistar rats, young diabetes-prone BB rats without insulitis or in diabetes-resistant BB rats. Immunohistochemistry of pancreatic sections using an iNOS-specific antiserum labeled the pancreas of adult diabetes-prone BB rats but not Wistar rats. Parallel staining for ED1-positive macrophages showed restriction of iNOS expression to areas of islet infiltration by macrophages. In conclusion, the data provide direct evidence for enhanced expression of inducible NO synthase in tissue lesions during the development of autoimmune diabetes.

publication date

  • August 9, 1993

Research

keywords

  • Amino Acid Oxidoreductases
  • Diabetes Mellitus, Type 1
  • Gene Expression Regulation, Enzymologic
  • Pancreas
  • Protein Biosynthesis
  • Transcription, Genetic

Identity

Scopus Document Identifier

  • 0027164671

Digital Object Identifier (DOI)

  • 10.1016/0014-5793(93)80954-s

PubMed ID

  • 7688327

Additional Document Info

volume

  • 328

issue

  • 1-2