An improved method detects differential NGF and BDNF gene expression in response to depolarization in cultured hippocampal neurons. Academic Article uri icon

Overview

abstract

  • Differential regulation of individual neurotrophins by impulse activity potentially allows transformation of instantaneous signalling into diverse, long-lasting neural alterations. To define the temporal profiles of trophin gene expression we examined nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) mRNAs in dissociated cell cultures of rat hippocampus using an improved solution hybridization technique. Traditional methods lack the precision and sensitivity to detect small changes during brief intervals and the facility to process large sample numbers simultaneously. This improved method has now allowed us to better define the dynamics of depolarization-induced changes in expression of individual trophin genes. Using elevated K+ as a depolarizing stimulus, NGF mRNA increased 40% after 48 h. In contrast, BDNF message rose almost 4-fold within 3 h and attained a maximal 6-fold increase within 6 h. Similar increases in BDNF mRNA levels were exhibited following treatment of cultures with glutamate, an excitatory neurotransmitter. To document the sensitivity of BDNF mRNA to depolarizing conditions, we examined expression after K+ withdrawal. BDNF message began decreasing within one hour post-depolarization, and returned to basal levels after 6 h. Observations indicate that BDNF and NGF mRNAs are induced differentially in response to impulse activity; BDNF message is acutely responsive to ongoing changes, whereas NGF mRNA responds more slowly and sluggishly. The physiological implications of this differential regulation are discussed.

publication date

  • October 1, 1994

Research

keywords

  • Gene Expression
  • Hippocampus
  • Nerve Growth Factors
  • Nerve Tissue Proteins
  • Neurons

Identity

Scopus Document Identifier

  • 0028096645

Digital Object Identifier (DOI)

  • 10.1016/0169-328x(94)90077-9

PubMed ID

  • 7854070

Additional Document Info

volume

  • 26

issue

  • 1-2