Supraspinal delta opioid receptor mRNA levels are not altered in [D-Ala2]deltorphin II tolerant mice. Academic Article uri icon

Overview

abstract

  • Delta opioid receptor (DOR) mRNA levels were studied in mice rendered tolerant to [D-Ala2]deltorphin II by 4 days of repeated intracerebroventricular administration (10 micrograms, [tid]). ED50 determinations on day 5 revealed a 10-fold loss in [D-Ala2]deltorphin II potency with the tail-flick test. Utilization of a microdissection technique followed by quantitative solution hybridization of RNA extracts from mouse brain revealed mean levels of DOR mRNA ranging from 3.9 pg/micrograms RNA in the caudate-putamen to 0.4 pg/micrograms RNA in the cerebellum. DOR mRNA levels were not different when RNA extracts from tolerant and non-tolerant mice were compared. These data suggest that altered DOR mRNA levels are not one of the adaptive changes that occur with delta opioid ([D-Ala2]deltorphin II) tolerance.

publication date

  • December 15, 1994

Research

keywords

  • Enkephalins
  • RNA, Messenger
  • Receptors, Opioid, delta
  • Spinal Cord

Identity

Scopus Document Identifier

  • 0028569076

Digital Object Identifier (DOI)

  • 10.1002/jnr.490390608

PubMed ID

  • 7897702

Additional Document Info

volume

  • 39

issue

  • 6