PCM-1, A 228-kD centrosome autoantigen with a distinct cell cycle distribution. Academic Article uri icon

Overview

abstract

  • We report the identification and primary sequence of PCM-1, a 228-kD centrosomal protein that exhibits a distinct cell cycle-dependent association with the centrosome complex. Immunofluorescence microscopy using antibodies against recombinant PCM-1 demonstrated that PCM-1 is tightly associated with the centrosome complex through G1, S, and a portion of G2. However, late in G2, as cells prepare for mitosis, PCM-1 dissociates from the centrosome and then remains dispersed throughout the cell during mitosis before re-associating with the centrosomes in the G1 phase progeny cells. These results demonstrate that the pericentriolar material is a dynamic substance whose composition can fluctuate during the cell cycle.

publication date

  • March 1, 1994

Research

keywords

  • Autoantigens
  • Cell Cycle
  • Cell Cycle Proteins
  • Organelles

Identity

PubMed Central ID

  • PMC2119948

Scopus Document Identifier

  • 0028353367

Digital Object Identifier (DOI)

  • 10.1083/jcb.124.5.783

PubMed ID

  • 8120099

Additional Document Info

volume

  • 124

issue

  • 5