Adeno-associated viral vector gene transfer into leptomeningeal xenografts. Academic Article uri icon

Overview

abstract

  • Leptomeningeal carcinomatosis is a painful and debilitating complication of cancer. Indwelling reservoirs provide continuous assess to the subarachnoid space, making leptomeningeal cancer potentially amenable to gene therapy. Adeno-associated virus (AAV) is a defective virus not associated with any human disease. We used an AAV vector to transduce medulloblastoma (DAOY) cells in a nude rat model of leptomeningeal disease. After intraventricular injection of vector carrying the bacterial lacZ gene, beta-galactosidase positive cells were found in the implanted tumor and in ependymal and subependymal cells but not in underlying normal brain parenchyma. No evidence of virally-mediated toxicity was noted in the animals. The results of this pilot study demonstrate that AAV vectors may be used to transfer and express foreign genes in established leptomeningeal tumors.

publication date

  • September 1, 1997

Research

keywords

  • Cerebellar Neoplasms
  • Gene Transfer Techniques
  • Genetic Therapy
  • Medulloblastoma
  • Meningeal Neoplasms
  • Subarachnoid Space

Identity

Scopus Document Identifier

  • 0030981077

Digital Object Identifier (DOI)

  • 10.1023/a:1005702228721

PubMed ID

  • 9210060

Additional Document Info

volume

  • 34

issue

  • 2