Regulation of Wnt signaling by Sox proteins: XSox17 alpha/beta and XSox3 physically interact with beta-catenin. Academic Article uri icon

Overview

abstract

  • Using a functional screen in Xenopus embryos, we identified a novel function for the HMG box protein XSox17 beta. Ectopic expression of XSox17 beta ventralizes embryos by inhibiting the Wnt pathway downstream of beta-catenin but upstream of the Wnt-responsive gene Siamois. XSox17 beta also represses transactivation of a TCF/LEF-dependent reporter construct by Wnt and beta-catenin. In animal cap experiments, it both activates transcription of endodermal genes and represses beta-catenin-stimulated expression of dorsal genes. The inhibition activity of XSox17 beta maps to a region C-terminal to the HMG box; this region of XSox17 beta physically interacts with the Armadillo repeats of beta-catenin. Two additional Sox proteins, XSox17 alpha and XSox3, likewise bind to beta-catenin and inhibit its TCF-mediated signaling activity. These results reveal an unexpected mechanism by which Sox proteins can modulate Wnt signaling pathways.

publication date

  • October 1, 1999

Research

keywords

  • Cytoskeletal Proteins
  • DNA-Binding Proteins
  • High Mobility Group Proteins
  • Proteins
  • Proto-Oncogene Proteins
  • Signal Transduction
  • Trans-Activators
  • Transcription Factors
  • Xenopus Proteins
  • Zebrafish Proteins

Identity

Scopus Document Identifier

  • 0033213619

Digital Object Identifier (DOI)

  • 10.1016/s1097-2765(00)80200-2

PubMed ID

  • 10549281

Additional Document Info

volume

  • 4

issue

  • 4