Harnessing the ubiquitination machinery to target the degradation of specific cellular proteins. Academic Article uri icon

Overview

abstract

  • The functional characterization of a specific gene, or its protein product, often relies on assessing the consequences of its elimination, usually accomplished by gene knockout, ribozyme, antisense, or RNA-mediated interference (RNAi) technologies. The selective degradation of cellular proteins is mediated primarily by the ubiquitin-proteasome pathway. Manipulation of the ubiquitin-dependent proteolytic machinery to eliminate specific gene products at the protein level has been previously attempted with some success in vitro; however, the in vivo efficacy of this approach has not yet been achieved. Here we report successful engineering of the substrate receptor of a major ubiquitin-proteolytic machinery to direct the degradation of otherwise stable cellular proteins both in yeast and in mammalian cells.

publication date

  • September 1, 2000

Research

keywords

  • F-Box Proteins
  • Ligases
  • Molecular Biology
  • Retinoblastoma Protein
  • Ubiquitins

Identity

Scopus Document Identifier

  • 0033638333

Digital Object Identifier (DOI)

  • 10.1016/s1097-2765(00)00074-5

PubMed ID

  • 11030355

Additional Document Info

volume

  • 6

issue

  • 3