Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules. Academic Article uri icon

Overview

abstract

  • The cargo that the molecular motor kinesin moves along microtubules has been elusive. We searched for binding partners of the COOH terminus of kinesin light chain, which contains tetratricopeptide repeat (TPR) motifs. Three proteins were found, the c-jun NH(2)-terminal kinase (JNK)-interacting proteins (JIPs) JIP-1, JIP-2, and JIP-3, which are scaffolding proteins for the JNK signaling pathway. Concentration of JIPs in nerve terminals requires kinesin, as evident from the analysis of JIP COOH-terminal mutants and dominant negative kinesin constructs. Coprecipitation experiments suggest that kinesin carries the JIP scaffolds preloaded with cytoplasmic (dual leucine zipper-bearing kinase) and transmembrane signaling molecules (the Reelin receptor, ApoER2). These results demonstrate a direct interaction between conventional kinesin and a cargo, indicate that motor proteins are linked to their membranous cargo via scaffolding proteins, and support a role for motor proteins in spatial regulation of signal transduction pathways.

publication date

  • March 5, 2001

Research

keywords

  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • Kinesins
  • Signal Transduction

Identity

PubMed Central ID

  • PMC2198804

Scopus Document Identifier

  • 0035809914

Digital Object Identifier (DOI)

  • 10.1083/jcb.152.5.959

PubMed ID

  • 11238452

Additional Document Info

volume

  • 152

issue

  • 5