Transbilayer movement of fluorescent phospholipid analogues in the cytoplasmic membrane of Escherichia coli. Academic Article uri icon

Overview

abstract

  • We investigated the transmembrane movement of fluorescent labeled phospholipids in inverted inner membrane vesicles (IIMV) of Escherichia coli (E. coli) wild-type strain (MG1655), as well as in proteoliposomes reconstituted from detergent extracts of the IIMV. The transbilayer movement of 1-myristoyl-2-[6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]caproyl]-sn-glycero-3-phosphoethanolamine (M-C6-NBD-PE) and -phosphocholine (M-C6-NBD-PC) was measured by a fluorescence stopped-flow back-exchange assay. Both analogues were rapidly translocated across the IIMV membrane, with half-times of <1 min (outward movement) and approximately 3 min (inward movement). No flip-flop was detected in protein-free liposomes, but in IIMV-derived proteoliposomes flip-flop of M-C6-NBD-PE occurred similarly to IIMV and could be largely eliminated by proteinase K treatment.

publication date

  • April 30, 2002

Research

keywords

  • 4-Chloro-7-nitrobenzofurazan
  • Cytoplasm
  • Escherichia coli
  • Fluorescent Dyes
  • Intracellular Membranes
  • Lipid Bilayers
  • Phospholipid Transfer Proteins
  • Phospholipids

Identity

Scopus Document Identifier

  • 0037197657

Digital Object Identifier (DOI)

  • 10.1021/bi0118714

PubMed ID

  • 11969421

Additional Document Info

volume

  • 41

issue

  • 17