Mice cloned from olfactory sensory neurons. Academic Article uri icon

Overview

abstract

  • Cloning by nuclear transplantation has been successfully carried out in various mammals, including mice. Until now mice have not been cloned from post-mitotic cells such as neurons. Here, we have generated fertile mouse clones derived by transferring the nuclei of post-mitotic, olfactory sensory neurons into oocytes. These results indicate that the genome of a post-mitotic, terminally differentiated neuron can re-enter the cell cycle and be reprogrammed to a state of totipotency after nuclear transfer. Moreover, the pattern of odorant receptor gene expression and the organization of odorant receptor genes in cloned mice was indistinguishable from wild-type animals, indicating that irreversible changes to the DNA of olfactory neurons do not accompany receptor gene choice.

publication date

  • February 15, 2004

Research

keywords

  • Cloning, Organism
  • Embryo, Mammalian
  • Embryonic and Fetal Development
  • Olfactory Receptor Neurons
  • Totipotent Stem Cells

Identity

Scopus Document Identifier

  • 1542347544

Digital Object Identifier (DOI)

  • 10.1038/nature02375

PubMed ID

  • 14990966

Additional Document Info

volume

  • 428

issue

  • 6978