Using transcriptome sequencing to identify mechanisms of drug action and resistance. Academic Article uri icon

Overview

abstract

  • Determining mechanisms of drug action in human cells remains a major challenge. Here we describe an approach in which multiple-drug-resistant clones are isolated and transcriptome sequencing is used to find mutations in each clone. Further analysis of mutations common to more than one clone can identify a drug's physiological target and indirect resistance mechanisms, as indicated by our proof-of-concept studies of the cytotoxic anticancer drugs BI 2536 and bortezomib.

publication date

  • February 12, 2012

Research

keywords

  • Antineoplastic Agents
  • Cell Cycle Proteins
  • Drug Resistance, Neoplasm
  • Protein Serine-Threonine Kinases
  • Proto-Oncogene Proteins
  • Sequence Analysis, DNA
  • Transcriptome

Identity

PubMed Central ID

  • PMC3281560

Scopus Document Identifier

  • 84857190127

Digital Object Identifier (DOI)

  • 10.1038/nchembio.779

PubMed ID

  • 22327403

Additional Document Info

volume

  • 8

issue

  • 3