Isolation and characterization of a cDNA clone encoding avian skeletal muscle C-protein: an intracellular member of the immunoglobulin superfamily. Academic Article uri icon

Overview

abstract

  • C-protein is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle A bands. Its function is unknown. To improve our understanding of its primary structure, we undertook the molecular cloning of C-protein mRNA. We describe the isolation and characterization of a cDNA clone, lambda C-86, that encodes approximately 80% of the fast isoform of C-protein in the chicken. Sequence analysis of the insert revealed that C-protein, although an intracellular, nonmembrane-associated protein, is a member of the immunoglobulin superfamily. Like several cell surface adhesion molecules that belong to this superfamily, C-protein contains sequence motifs that resemble immunoglobulin domains and fibronectin type III repeats. Computer searches using the C-protein sequence also lead to the identification of related domains in chicken smooth muscle myosin light chain kinase that have not been reported previously.

publication date

  • March 1, 1990

Research

keywords

  • Genes, Immunoglobulin
  • Multigene Family
  • Muscle Proteins
  • Muscles

Identity

PubMed Central ID

  • PMC53645

Scopus Document Identifier

  • 0025246301

Digital Object Identifier (DOI)

  • 10.1073/pnas.87.6.2157

PubMed ID

  • 2315308

Additional Document Info

volume

  • 87

issue

  • 6