Dopamine- and cAMP-regulated phosphoprotein (DARPP-32) and dopamine DA1 agonist-sensitive Na+,K+-ATPase in renal tubule cells. Academic Article uri icon

Overview

abstract

  • The cellular localization of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein of Mr 32,000 that appears to mediate certain actions of dopamine in the mammalian brain by acting as an inhibitor of protein phosphatase 1, was studied in the kidney of several species. DARPP-32 mRNA and DARPP-32-like immunoreactivity were found in the cytoplasm of cells in the thick ascending limb of the loop of Henle. The specific dopamine DA1 agonist SKF 82526 caused a dose-dependent inhibition of Na+,K+-ATPase activity, which could be blocked by SCH 23390, a specific DA1 antagonist, and by PKI-(5-24) amide, a specific inhibitor of cAMP-dependent protein kinase. The results indicate that DA1 dopamine receptors and DARPP-32, an intracellular third messenger for dopamine, are part of the signal-transduction process for dopamine acting on renal tubule cells.

publication date

  • October 1, 1989

Research

keywords

  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
  • Dopamine Agents
  • Kidney Tubules
  • Nerve Tissue Proteins
  • Phosphoproteins
  • Sodium-Potassium-Exchanging ATPase

Identity

PubMed Central ID

  • PMC298216

Scopus Document Identifier

  • 0344791242

Digital Object Identifier (DOI)

  • 10.1073/pnas.86.20.8068

PubMed ID

  • 2573060

Additional Document Info

volume

  • 86

issue

  • 20