flippant-An R package for the automated analysis of fluorescence-based scramblase assays. Academic Article uri icon

Overview

abstract

  • BACKGROUND: The lipid scrambling activity of protein extracts and purified scramblases is typically measured using a fluorescence-based assay. While the assay has yielded insight into the scramblase activity in crude membrane preparations, functional validation of candidate scramblases, stoichiometry of scramblase complexes as well as ATP-dependence of flippases, data analysis in its context has remained a task involving many manual steps. RESULTS: With the extension package "flippant" to R, a free software environment for statistical computing and graphics, we introduce an integrated solution for the analysis and publication-grade graphical presentation of dithionite scramblase assays and demonstrate its utility in revisiting an originally manual analysis from the publication record, closely reproducing the reported results. CONCLUSIONS: "flippant" allows for quick, reproducible data analysis of scramblase activity assays and provides a platform for review, dissemination and extension of the strategies it employs.

publication date

  • March 3, 2017

Research

keywords

  • Biochemistry
  • Lipids
  • Phospholipid Transfer Proteins
  • Software

Identity

PubMed Central ID

  • PMC5335725

Scopus Document Identifier

  • 85014380339

Digital Object Identifier (DOI)

  • 10.1128/EC.00198-07

PubMed ID

  • 28253836

Additional Document Info

volume

  • 18

issue

  • 1