XLF and H2AX function in series to promote replication fork stability. Academic Article uri icon

Overview

abstract

  • XRCC4-like factor (XLF) is a non-homologous end joining (NHEJ) DNA double strand break repair protein. However, XLF deficiency leads to phenotypes in mice and humans that are not necessarily consistent with an isolated defect in NHEJ. Here we show that XLF functions during DNA replication. XLF undergoes cell division cycle 7-dependent phosphorylation; associates with the replication factor C complex, a critical component of the replisome; and is found at replication forks. XLF deficiency leads to defects in replication fork progression and an increase in fork reversal. The additional loss of H2AX, which protects DNA ends from resection, leads to a requirement for ATR to prevent an MRE11-dependent loss of newly synthesized DNA and activation of DNA damage response. Moreover, H2ax-/-:Xlf-/- cells exhibit a marked dependence on the ATR kinase for survival. We propose that XLF and H2AX function in series to prevent replication stress induced by the MRE11-dependent resection of regressed arms at reversed replication forks.

publication date

  • May 23, 2019

Research

keywords

  • DNA-Binding Proteins
  • Histones
  • MRE11 Homologue Protein

Identity

PubMed Central ID

  • PMC6605786

Scopus Document Identifier

  • 85069264585

Digital Object Identifier (DOI)

  • 10.1126/science.1083430

PubMed ID

  • 31123184

Additional Document Info

volume

  • 218

issue

  • 7