Accurate initiation at RNA polymerase II promoters in extracts from Saccharomyces cerevisiae. Academic Article uri icon

Overview

abstract

  • A yeast nuclear extract supported transcription from the CYC1 and PYK1 promoters. Transcription was initiated in vitro at or near sites used in vivo. Deletion of "TATA" sequences abolished the reaction. alpha-Amanitin (10 micrograms/ml) and chloride (100 mM) were highly inhibitory.

publication date

  • December 1, 1987

Research

keywords

  • Promoter Regions, Genetic
  • RNA Polymerase II
  • Saccharomyces cerevisiae
  • Transcription, Genetic

Identity

PubMed Central ID

  • PMC299646

Scopus Document Identifier

  • 0023515307

Digital Object Identifier (DOI)

  • 10.1073/pnas.84.24.8839

PubMed ID

  • 3321057

Additional Document Info

volume

  • 84

issue

  • 24