High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements. Academic Article uri icon

Overview

abstract

  • Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells.

publication date

  • October 1, 1979

Research

keywords

  • Cytoskeleton
  • Microtubules

Identity

PubMed Central ID

  • PMC2110435

Scopus Document Identifier

  • 0018572272

Digital Object Identifier (DOI)

  • 10.1083/jcb.83.1.249

PubMed ID

  • 574514

Additional Document Info

volume

  • 83

issue

  • 1