E-cadherin distribution in interleukin 6-induced cell-cell separation of ductal breast carcinoma cells. Academic Article uri icon

Overview

abstract

  • E-cadherin is expressed in both the ZR-75-1-Tx and the ZR-75-1-Ro sublines of ductal breast carcinoma cells and is concentrated at cell-cell borders as shown by immunocytochemical examination. Free cell borders generally show no or little staining. The localized decrease in E-cadherin expression observed after interleukin 6 (IL-6) treatment of either subline correlates with the increase in free cell borders as IL-6 causes cell-cell separation. As we previously reported, many IL-6-treated ZR-75-1-Tx cells round up and detach from the substratum while ZR-75-1-Ro cells remain adherent and display prominent processes. The results are consistent with the view that E-cadherin expression is not responsible for the marked difference in the IL-6-induced phenotypes in these cell lines, although the localized decrease may play a role in cell-cell separation. ZR-75-1-Tx cells are deficient in desmosomes and show a wider intercellular space than ZR-75-1-Ro cells. Alternative mechanisms involving different aspects of the interlinked cytoskeletal and cell adhesion structures are considered to account for the IL-6-induced antimorphogenetic effect.

publication date

  • May 10, 1994

Research

keywords

  • Breast Neoplasms
  • Cadherins
  • Carcinoma, Ductal, Breast
  • Cell Adhesion
  • Interleukin-6

Identity

PubMed Central ID

  • PMC43780

Scopus Document Identifier

  • 0028345171

Digital Object Identifier (DOI)

  • 10.1073/pnas.91.10.4338

PubMed ID

  • 8183909

Additional Document Info

volume

  • 91

issue

  • 10