Specific antibodies to the external vestibule of voltage-gated potassium channels block current. Academic Article uri icon

Overview

abstract

  • Using delayed-rectifier potassium channels as examples, we have designed two specific blockers by generating specific antipeptide antibodies to epitopes in the external vestibules of two channel proteins, Kv1.2 and Kv3.1. These antibodies reduced whole-cell Kv1.2 or Kv3.1 currents in transfected cells and the effect was blocked by the corresponding peptide antigen, but not by control peptides. A control antibody had little effect on Kv1.2 currents and the Kv1.2 blocker antibody had limited effect on other related potassium currents. Furthermore, the Kv1.2 blocking antibody inhibited dendrotoxin binding to Kv1.2 channel proteins in transfected cells. Moreover, using the Kv1.2 blocker antibody, we determined the presence and relative contribution of endogenous Kv1.2 to the overall endogenous K+ currents in NG108 neuronal cells. This guided design of specific channel blockers will facilitate future physiological studies on ion channel functions.

publication date

  • April 1, 1998

Research

keywords

  • Antibodies
  • Ion Channel Gating
  • Potassium Channel Blockers
  • Potassium Channels

Identity

PubMed Central ID

  • PMC2217123

Scopus Document Identifier

  • 0031900727

Digital Object Identifier (DOI)

  • 10.1085/jgp.111.4.555

PubMed ID

  • 9524138

Additional Document Info

volume

  • 111

issue

  • 4